Four protein fractions of Osborn classification system explain the relative per cent of particular protein based on total protein. Albumin fraction was varied from 2.24 to 4.63% of the total protein in maize seed, globulin showed range of 2.78 to 5.61% of the total protein, whereas glutelin showed 33.81 to 41.50% of the total protein. The prolamin fraction exhibited 57.58 to 66.35 per cent on the basis of total protein. These results showed the albumin, globulin and glutelin fractions were higher for QPM lines whereas the prolamine fraction was lower for the QPM and higher for normal lines, as prolamine is limiting for lysine and tryptophan content. Further when studying fourth alcohol soluble Zein fraction, they exhibit a range of variable bands for twenty inbred lines, could group the maize lines into two main, QPM and normal maize lines. From these findings, electrophoresis of zeins can be used as a tool for selecting good modifier background in QPM maize breeding. Among four fractions, albumin globulin and glutelin content showed positive correlation and higher per cent for QPM lines while prolamine showed negative correlation with others and was significantly lower for QPM lines which clearly reflect the reason for higher lysine and tryptophan content in QPMs.